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جب بھی درپیش مصیبت کی گھڑی ہوتی ہے

جب بھی درپیش مصیبت کی گھڑی ہوتی ہے
آپؐ کی ذات مرے ساتھ کھڑی ہوتی ہے

جب خیالوں میں مضافاتِ مدینہ آئیں
چشم کے صحن میں اشکوں کی جھڑی ہوتی ہے

تیرہ بختوں سے کہو سر کو جھکا کر دیکھو
روشنی آپؐ کے قدموں میں پڑی ہوتی ہے


ذکرِ احمدؐ ہی سے تسکین ملی ہے اُس دم
جب کبھی ذات مری مجھ سے لڑی ہوتی ہے

درسِ اُمید ملا شہؐ سے کچھ ایسے کہ سدا
اک کرن سینۂ ظلمت میں گڑی ہوتی ہے

آپؐ کی شان تو بڑھتی ہے مسلسل عابدؔ
جب بھی دُشمن نے کوئی بات گھڑی ہوتی ہے

Return Migration to Pakistan during COVID19 Pandemic: Unmaking the Challenges

In order to contain the spread of corona virus (COVID-19) disease, strict border closure measures have been taken globally. Migrants and refugees are affected across the globe due to such measures. Amid the COVID-19 pandemic, the economic recession escalated across the globe which is expected to have serious implications for the migrant workers and laborers. Potohar region was selected to explore implications of return migration from abroad, but the fieldwork halted immediately due to the pandemic outbreak. Since the data on Pakistani migrants is dispersed and scant, it was nearly impossible to stick to the locale for returnees. Therefore, the data for this study was collected qualitatively using exploratory methodology. In-depth interviews were conducted using interview guide as a tool of research. The paper is based on analysis of the narratives based on the experiences of the returnees especially. It sheds light on the state of the stranded Pakistani migrants who have been returning or awaiting repatriation due to layoffs. It delves into long-run and short-run challenges due to return migration, in Pakistan which highly depends on remittances from abroad. Challenges created by sudden end to remittances, entrepreneurial setups and returnees’ reintegration in the society need immediate attention. It is recommended that the state should support and monitor the migrants living abroad, ease out issues in repatriation of the laid off workers, pave way for investment, offer insurance plans, reduce reliance on remittance flow and engage them in Public Private Partnerships for sustainable reintegration.

Molecular and Physiological Evaluation of Wheat Cultivars for Drought Tolerance

Wheat is grown in rainfed areas characterized by erratic rainfall with a high probability of drought spells during the vegetative and reproductive growth. Drought poorly affects crop growth and yield by altering metabolic and physiological processes. Plants tolerate drought through osmotic adjustment, stomatal resistance, increased water uptake and accumulation of waxy layer. Cultivars possessing drought tolerant traits may be promising candidates for drought prone regions. The present study was planned for molecular, physiological and biochemical evaluation of wheat cultivars for drought tolerance. Four experiments were accompanied for this purpose. In first experiment wheat seeds of five cultivars (GA-2002, Chakwal-97, Uqab- 2000, Chakwal-50 and Wafaq-2001) were exposed to osmotic stress (-2, -4, -6, and -8 bars) created by soaking the blotters with different concentrations of PEG-6000. Assessment of cultivars was based on germination percentage, speed of germination, coleoptile length, root-shoot length, dry and fresh weight. In second laboratory experimentation, sterilized seeds were sown in petri plates and after seven days the seedlings were transplanted into hydroponics having modified MS medium solution followed by its supplementation by PEG-6000 to induce osmotic stress of -2, -4, -6 and -8 bars at 3-4 days interval. After two days, plants exhibited visible effects of stress. Thus the plants were harvested for analysis and data collection. Evaluation of cultivars was constructed on proline, sugar, chlorophyll, protein, amino acids, root- shoot dry and fresh weight basis. All cultivars exhibited decline in germination percentage, speed of germination, root-shoot length, coleoptile length, seedling fresh and dry weight under water stress environments. Total soluble sugar, soluble protein, amino acids and proline have direct relationship with osmotic stress while total chlorophyll contents, seedling fresh and dry weight have inverse association with water stress. Third experiment was carried out by employing different levels of field capacity (80%, 60%, 40% and 20%) as stress sources in glass house/plant shed. Cultivars were assessed on the basis of Gas exchange (photosynthesis rate, transpiration rate and stomatal conductance) measurements, epicuticular wax, relative water content and osmotic potential. With increase in water stress photosynthesis rate, transpiration rate, stomatal conductance, relative water content and osmotic potential drops however epicuticular wax content increased with an increase in water stress conditions. At the end for drought response, wheat cultivars were evaluated on molecular basis. A set of specific primers (TdDHN15, WDHN13, TdDHN16, TdDHN9.6) were used for presence and expression of dehydrin genes which can confer drought resistance to a genotype. RNA was extracted by Trizol method. Then first strand cDNA synthesis was done by using superscript II RT. PCR was carried out by using the cDNA. PCR products were separated on Agrose gel and visualized under gel doc system. Chakwal-50 followed by GA-2002 and Chakwal-97 performed best at various stress levels for germination percentage, speed of germination, root-shoot fresh and dry weight, total chlorophyll contents, proline, soluble protein, total soluble sugars contents, photosynthesis rate, transpiration rate, stomatal conductance, wax content, osmotic potential and relative water contents. However performance of Wafaq-2001 and Uqab-2000 was poor. The gene expression of WDHN13 was observed only in GA- 2002 and Chakwal-50. None of the other gene expression was recorded in any other cultivars. It can be concluded that water stress levels had substantial effects on germination and seedling growth. Morphological, biochemical, physiological and molecular analysis revealed that adequate genetic difference for drought tolerance existed in wheat genotypes tested. Chakwal-50 and GA-2002 may prove a promising parent material for breeding drought tolerant wheat cultivars. Further molecular investigations are suggested to assess the genetic basis of drought tolerance. Chakwal- 50 and GA-2002 may be considered better genotypes for low rainfall drought prone areas.
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