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نواب علی یاور جنگ بہادر

نواب علی یاورجنگ بہادر
گزشتہ ماہ دسمبر میں نواب علی یاورجنگ بہادر گورنر بمبئی کا انتقال ہوگیا۔ ۱۹۴۷؁ء کے بعد حکومت ہند کی طرف سے ان کو بڑے بڑے عہدے ملتے رہے، وہ امریکہ میں ہندوستان کی طرف سے سفیر بناکر بھیجے گئے، مسلم یونیورسٹی علی گڑھ کے وائس چانسلر بنائے گئے، بمبئی کی گورنری کے عہدے پر مامور ہوئے اور اسی ریاست کے گورنر کی حیثیت ہی سے عالم بقا کو سدھارے، اور معلوم نہیں کتنے دوسرے اعزاز ان کو حاصل ہوتے رہے، حکومت ہند کے معتمد ترین حکام میں ان کا شمار ہوتا رہا۔
میں نے ان کو کسی بڑے عہدیدار کی حیثیت سے نہ جانا اور نہ پہچانا، بلکہ ان سے نواب عمادالملک کے نواسے کی حیثیت سے ملتا رہا، نواب عماد الملک دارالمصنفین کی مجلس انتظامیہ کے پہلے صدر تھے، جن کا احسان دارالمصنفین پر بہت بڑا تھا، ان ہی کی مساعی جمیلہ سے علامہ شبلیؒ کی وفات کے بعد ان کا ماہانہ وظیفہ دارالمصنفین کے نام منتقل ہوا، جس سے اس کی تاسیس میں بڑی مدد ملی، وہ دارالمصنفین کے بڑے قدرداں اور سرپرست رہے، جب ان کی وفات ۳؍ جون ۱۹۲۶؁ء کو ہوئی تو استاذی المحترم مولانا سید سلیمان ندویؒ نے معارف کے شذرات میں اپنی غیرمعمولی سوگواری کا اظہار کیا، جس میں ان کے اور بہت سے فضائل اور محاسن کے ساتھ مولانا شبلی، دارالمصنفین اور خود ان سے جو تعلقات رہے، اس کا ذکر بہت ہی خوش عقیدگی سے کیا، جس کے کچھ ٹکڑے یہ ہیں:
’’آخر عمر میں مولانا شبلی مرحوم کی تحریک سے انھوں نے قرآن مجید کا انگریزی میں ترجمہ شروع کیا تھا جو سولہ پاروں تک ضعف بصارت و علالت کی وجہ سے رک گیا، اس ترجمہ میں بالکل بائبل کی زبان اختیار کی ہے‘‘۔
’’مولانا شبلی مرحوم سے ان کا تعلق سرسید کے زمانہ...

IS REGULATORY EMOTION SELF-EFFICACY PLAYING A MEDIATING ROLE? IMPACT OF LIFESTYLE HABITS ON SUBJECTIVE WELL-BEING

Background and Aim: The objective of the research is to investigate the impact of lifestyle habits on subjective wellbeing in the presence of role of regulatory emotion self-efficacy as a mediator. Vital for wellbeing, longevity, productivity, relationships, and general quality of life in all areas is a healthy lifestyle. Methodology: A cross-sectional study has been conducted for 271 Students had been selected as respondents. Smart Pls has been used for calculating the reliability, validity of questionnaire and as well as hypothesis testing. Results: Food disorder, healthcare anxiety, regulatory emotional self-efficacy have direct and indirect significant impact on subjective well-being. But sleeping disorder has showed the direct and indirect insignificant impact on subjective well-being. Limitation and Future Implications: Because of time constraints, a lack of comprehension of the research, and a healthy lifestyle people are not aware of health benefits, they had less knowledge about it and were hesitant about a discussion. Originality: The role of regulatory emotion self-efficacy has not been used as a mediator during the relationship of lifestyle habits and subjective well-being. Conclusion: Subjective well-being is defined as gratification and satisfaction with one’s life. Regular physical activity is important in order to enhance the self-efficacy and emotional well-being. Good nutrients and a balanced diet provide a person with enough energy to carry out the tasks of daily life effectively.

In Vitro Regeneration and Agrobacterium Mediated Transformation of Soybean Glycine Max L.

Agrobacterium mediated transformation of soybean has successfully been achieved. However, the efficiency is usually low indicating that Agrobacterium mediated transformation of soybean demands optimization of more suitable conditions for transfer of T-DNA from plasmid to plant cell; beside these constraints; transformation is also dependent upon plant genotype, Agrobacterium strain and type of plasmid. Present investigation was aimed to find suitable regeneration protocol for two soybean cultivars, NARC-4 and NARC-7 and to standardize some transformation conditions. Based on optimized regeneration and transformation scheme, LFY gene, for early flowering and rol genes (A, B & C) for enhanced rooting were introduced in soybean genome. For transfer of LFY gene EHA105 harboring pROKIILFYGUSintnptII and for rol gene, LBA4404 harboring pLBR were used. Cotyledonary node method was found suitable for direct organogenesis of soybean NARC-4 and NARC-7 cultivars. However, presence of different plant growth regulators in media resulted in variation in number of shoot produced, shoot length and in percentage response. Statistical analysis reveals that BAP resulted in higher frequency of shoot regeneration and number of shoots per explant while mean shoot length was found higher when ZTR was used in the medium. Out of both soybean cultivars, NARC-4 showed better response than NARC-7. For standardization of transformation conditions, different parameters were studied. It was observed that explant cutting in Agro-suspension culture and infection for 1hr resulted in the highest GUS expression (48.3% and 55.9%, respectively). While 5 days co-cultivation resulted in 55.17% GUS response and washing for 2hr in washing medium containing 1g/L cefotaxime was better. It was found that 30mg/L kanamycin was sufficient in selection medium. At this concentration number of GUS positive shoots were maximum (63.6% response). The overall percentage transformation efficiency of both soybean cultivars NARC- 4 and NARC-7 was 24.16% and 15.71% respectively, when tested at best conditions. 11Soybean cultivar NARC-4 was used for transformation of LFY and rol genes. EHA 105 containing LFY gene along with GUS as reporter and nptII as selectable marker showed transformation efficiency 2.2%. Only 4 plants were recovered and confirmed for presence of LFY gene by PCR. These plants were highly dwarfed with small leaves and short nodal distance. Early flowering was observed by these transforments. The flowers turned brown and died after few days. Few of them turned into pods but no seed formation was observed. All the rol transforments produced enhanced rooting as compared to control plants. However, plants morphology varied depending upon rol gene. RolA transforments were small in size and mildly shrubby with ovate to elliptical leaf shape while rolC transforments were also dwarf with divided stem at the base with ovate to slightly globular leaf shape. Soybean rolB transformed plants showed variation in morphology. These plants were dwarf to shrubby with variation in leaf shape. The shrubby plants had reduced nodal distance with a little more zigzag pattern as compared to non-transformed plants. Rol transforments produced flowers in less time period as compared to control plants. These flowers converted into pods and set seeds. PCR analysis confirmed presence of respective gene in these plants. Southern blot analysis confirmed insertion of T-DNA in soybean genome as single copy number to multiple copies in rol gene transforments. In this study rol and LFY genes were efficiently introduced in soybean cultivar NARC-4 after optimization of regeneration and some transformation conditions.
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