Search from the Journals, Articles, and Headings
Advanced Search (Beta)
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

۱۔ دستِ فراخ

دست ِ فراخ

میں وہ تیرگی ہوں

 جس کے واسطے

آسماںکو چیرتے

اک نورِ عظیم سے

دشت کی وسعتیں چمک اٹھیں

پہاڑوں کو چوٹیاں دمک اٹھیں

میں وجہِ قیام ِطویل ہوں

کہ شب بھی رو پڑے

 خدا بھی پکار اٹھے

 بس کیجیے !بس کیجیے

میں وہ خاکِ خوش نصیب ہوں

جس پہ تحائفِ سماوی کا نزول ہے

میں آنسوئوں سے تروہ دعا ہوں

جسے ازل سے اندیشہ ٔ رد نہیں

جو فقط قبول ہے،قبول ہے

 میں وہ غم ِ بختیار ہوں

جسے دلِ اطہر کی پناہ ملی

 وہ راہ نور ہوں

جسے روشن نگاہ ملی

بس اب اتنی ہے آرزو

پاک فضا میں سانس لوں

زمزم میرا مشروب ہو

سایۂ سبز تلے پڑا رہوں

اور جب ہو عالمِ تشنگی

 ساقیِ دو جہاں کے دستِ فراخ سے

وہ جامِ تمنا نصیب ہو

جس کی سدا تمنا رہی

Detection of Metalo-Beta-Lactamase Gene in Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Lahore, Pakistan Detection of Metalo-Beta-Lactamase Gene in Pseudomonas Aeruginosa

Pseudomonas aeruginosa is a widespread organism, caused severe nosocomial infection in human and associated with multiple drug resistance (MDR)Objective: The present study was carried out to observe current antimicrobial resistant pattern of Pseudomonas aeruginosa in Lahore and to detect the Metallo-beta-lactamase (MBL) gene in carbapenem resistantPseudomonas aeruginosaMethods: By screening 360 samples total 123 Pseudomonas aeruginosa was identified by standard microbiology techniques such as microscopy and biochemical testing. The isolated Pseudomonas aeruginosa was evaluated for drug resistance by disc diffusion method and polymerase chain reaction(PCR) was used to identify the carbapenem resistance causing gene (bla-VIM and bla-IMP) Results: Following antibiotic resistant pattern was observed, Gentamycin (59.00%), Ceftazidime(58.7%), Ceftriaxone (58.00%), Cefotazime (57.0%) and Ciprofloxacin (55.00%). Resistance rates to carbapenem group of antibiotics is Doripenem (30.5%) Meropenem(31.0%) and Imipenem (28.0%). Out of 123 samples of Pseudomonas aeruginosa, 28 isolates were found resistant to carbapenem group of antibiotic which was supposed to be highly sensitive for this bacterium. Molecular based identification of resistance genes showed that bla-IMP gene was present in 32.1% (09) and bla-VIM was found positive in 17.8% (04) samples. Metallo-beta-lactamasesproducing genes (bla-VIM and bla-IMP), amongcarbapenem resistant Pseudomonas aeruginosa were detectedin 28.1% of samples. If other carbapenem resistant gene were also included this number might be higherConclusions: PCRbased test should be included in routine laboratory examination for quick detection of the resistancecausing genes.

Allelopathy of Medicinal Plants and its Impact on Conservation of Soil Environment

The taxonomic richness of medicinal plants in Pakistan can foster notable economic contributions through medicinal, industrial and environmental applications. The release of allelochemicals from medicinal plants have metamorphosed the discourse of research on medicinal plants. The allelopathy epitomizes stimulatory or inhibitory interactions among organisms due to their inherent ability to forego biological invasions and impede biological activities by release of certain chemicals. This study extended to the allelopathic effect of 221 species collected from different regions of Pakistan. The cardinal objective was to assess the allelopathic effect of medicinal plants collected from different regions of Pakistan using dish pack method. As the dish pack method allows the assessment of allelopathic effect due to volatile secondary metabolites released from different plants. Hence, the germination and growth of lettuce was tested against selected plant species. The findings of this study envisaged the Boerhavia procumbens for strong inhibitory effect and Plectranthus rugosus for strong stimulatory effect due to release of volatile allelochemicals. The results hereby envisions future applications of allelopathy in sustainable agriculture, industry, weed management, agro-environment conservation, food security and soil conservation. The second objective of this study was to explore allelopathic effect of medicinal plants on the germination and seedling growth of lettuce using sandwich method. The 207 plant species had been tested using sandwich method to assess allelopathic effect of leachates of different parts of plants on lettuce seeds and provide baseline information for exploration of medicinal plants for economic benefits. The results of this study identifies 4 plants having strong inhibitory effect, 14 plants with medium inhibitory and 13 plants of low inhibitory effect on the lettuce seeds. Boerhavia procumbens exhibited the strongest inhibitory allelopathic effect and Viburnum grandiflorum presented strong stimulatory effect on the growth of lettuce seeds. The allelopathic effects of aqueous extract of different plant species on wheat (Triticum aestivum L.), Lettuce (Lactuca sativa L.) and maize (Zea mays L.) were also assessed in this study. We have selected nine plant species (Boerhavia procumbens Banks ex Roxb., Jacaranda mimosifolia D. Don, Datura metel L., Asparagus adscendens Roxb., Plectranthus rugosus Wall. ex Benth. Parthenium hysterophorus L., Arisaema triphyllum (L.) Schott, Crotalaria medicaginea DC. and Amaranthus viridis L) and apply their aqueous extract directly to crop seeds as well as through the soil medium to assess their allelopathic effect in both conditions. The results apprehended that the aqueous extracts of all the selected species revealed significant inhibitory effect on germination and growth of wheat, maize and lettuce as compared to the aqueous extracts applied to the soil. However, lettuce was more influenced from the inhibitory effect followed by maize and wheat in the soil irrigated with the aqueous extracts of selected species. Hence, the diversified allelopathic effects of plants have the potential to improve weed management, sustainable agriculture, food production as well as medicinal, industrial and environmental applications
Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.