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سلام

سلام
جب شفق کے کانوں میں بالیاں چمک رہی تھیں
آباد حویلیوں کی سنہری جالیاں چمک رہی تھیں
طاق میں روشن چراغوں کو۔۔۔!
کھڑکیوں اور دروازوں کو۔۔۔!
صفاء اور مروا کی چوٹیوں کو چوم کر۔۔۔!
ہوائیں عقیدت میں جھک کر سلام کرتی تھیں
پھر شفق کی چمکتی بالیوں کے۔۔۔!
سنہری چمکتی جالیوں کے روبرو۔۔۔!
باوفا قافلے کی تشکیل میں!
قافلے کا سارباں ،رازداں چنا گیا
سوسن و نسترن کا گلابی قرار چنا گیا
با وفا صحیفے کے ساتھ۔۔۔خیمہ بھی۔۔۔!
ماہتابی شباب، چراغ اور ردا بھی تھی
پھر ظہر ہوئی۔۔۔عصر بھی گزر گئی
اب تو دشت نینواء پر۔۔۔!
سوسن و نسترن کے پھول بکھرے ہوئے ہیں
جلتے خیموں کا دھواں ہی دھواں ہے
اے حسینؑ اب علیؑ تجھ پر سلام
اے بنتِ حسینؑ و علیؑ تجھ پر سلام

ڈیرہ غازی خان کی مذہبی و معاشرتی زندگی میں صوفیا کرام کی تعلیمات کے اثرات

Islam is the religion of peace and love for the humanity. It always ordered to his followers to treat other with kind and tolerance. The Holy prophet always proved himself the prophet of mercy as the Holy Quran Said, but we sent you as a mercy to the worlds. Islam spread through politeness. In Subcontinent the advent of Islam is a great achievement of the noble saints and sufis. D۔G Khan which was a purely Hindu area in the past and the several sufis came to this part of Punjab in the initial stage of Islam. They played glorious role in the advent of Islam in the region. Sufism is a deter-mine effort towards unity. His main purpose is to bring humanity close with one another, separated as it is into so many different units, closer together in the deeper understanding of life. The main task is to bring about brotherhood among races, nations and faiths and to respect one another's faith, scripture and teacher. Sufi is to confer Sympathy on these lives, to impart Love, Compassion and Kindhearted-ness on all creations. The Sufi message is the resonance of the same Divine message which has always come and will always come to inform humanity. This research paper highlights the services of sufis who influenced on socio religious life of the people of D۔G Khan. 

Characterization and Metal Coordination Studies of Novel Metallo-B-Lactamase

Antibiotics are used since 1940 to fight bacterial infections due to versatility and high efficacy against bacteria. Metallo-β-lactamases (MBLs) are zinc ion dependent enzymes that are responsible for the emergence and spread of β-lactam resistance. Clinically useful MBL inhibitors are not yet available. The increasing number of novel members of this family is threatening to global health care. Sequence identities and structural characteristics at active site has led to the inception of MBL subclasses B1, B2, B3 and recently B4. AIM-1 (Adelaide IMipenemase-1) is a novel and poorly characterized MBL that was found in Adelaide, Australia and found to be similar in many ways to numerous other enzymes that pose a threat to human health because of their ability to protect bacteria from penicillin like compounds. Being an exclusive member of B3 subclass AIM-1 is suggested as a potential candidate for broad spectrum MBL inhibitor. In present study, a series of mutant forms of AIM-1 was produced by site-specific mutagenesis. D155, D220 and T223 were selected as second shell metal coordinated residues in case of Zn1 whereas D120 was selected as primary ligand. The native and mutant enzymes were then expressed and purified so that the kinetic properties of the mutant enzymes could be compared with those of the native enzyme. Metal chelators were used to remove the metals from the native and mutant forms of the enzyme so that ITC (isothermal titration calorimetry) could be used to determine the metal binding constants. Kinetic data suggest that Asp120 and second shell ligands have showed clear effects and are important for enzymatic activity. In case of Zn1 D to N mutagenesis in second metal coordinates accommodate the smaller substrate molecules like Carbapenems and can effect metal binding whereas D to A mutation severely impaired catalytic rate. Two exothermic metal binding events were observed where one is stronger and other is weaker. Later, two putative MBLs were identified by protein database search, in silico characterized and compared with AIM-1. Information gained from this study may also be useful in the design of inhibitors that could be used as anti-bacterial agents.
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