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۶۔ نکلنا خلد سے آدم کا

نکلنا خلد سے آدم کا۔۔۔

آس کی خوشبو یاس کی بد بو پرہمیشہ غالب آتی ہے

یوں محسوس ہو رہا تھا

 جیسے دل میں اِ ک آگ سی لگی ہو

اور سب کچھ اس بھٹی میں جھونک رہا ہوں

 اس بھٹی کو صرف آنسو ہی ٹھنڈا کر سکتے تھے 

لیکن !وہ بھی لب ِ مژگاں تک آتے آتے مر گئے

 کتنی مشکل سے محبت کے تارو پود سے سنہری خواب بنے تھے

مگرایک تار کے نکل جانے سے

سب خواب نیند کی وادیوں میں کہیں کھو گئے ہیں

حیف!میرے دھندلے خواب

 جو میں کسی کی آنکھوں سے دیکھ رہا تھا

 یوں لگتا ہے جیسے

میرا بھی شمار اُن سوختہ سروںمیں ہوتا ہے

جو بحرِ محبت میں بنا کشتی اور پتوار کے اتر گئے تھے

مجھے اپنی عادتوں پردکھ ہے

 جو پھر سے خراب ہو جائیں گی 

جنہیں اک عرصہ بدلنے میں لگا تھا

یہ آدم اور آدم زاد کے نصیب میں لکھا جا چکا ہے

کہ اس کا کبھی ایک ٹھکانہ ہو ہی نہیں سکتا

کبھی یہ بہشت سے نکالا جاتا ہے 

کبھی اپنے گھر سے دربدر ہوتا ہے تو کبھی شہر سے

کبھی کسی کی زندگی سے توکبھی کسی کے دل سے

یزداں نے بھی انسان کے ساتھ کیاخوب کھیل کھیلا ہے 

 اسے فاصلوں میںالجھائے رکھتا ہے

Grief and Shock, Discernment and Dificulties, Their Reasons and Motives in the Light of the Teachings of the Holy Prophet (PBUH)

Islam has this faith that everything of the universe, small or big has come into existence with the will, power, method and knowledge of Allah. Fate is the attribution of Allah, which is the truth. It is the condition to have belief of this faith. Since, faith is the attribution of Allah and it’s impossible for anyone to know the extent of godly attributions, so the issue of fate is delicate and precise. One must only have the faith that whatever is happening in the world was already in the knowledge of Allah and god has already saved it on the safe tablet. The dilemma of fate has not been solved by anyone and it will never be solved. There must be a simple faith that whatever is happening in the world has already known by Allah as the Creator of everything is God and everything has come into existence with His creation. All the matters happening in the world are of two kinds. In some of them the will and authority is prevail. In these matters which are to be done which are not to be done orders of their execution or prohibition are given. If they are not performed because of will and authority, there will be accountability for them. Secondly, all those works which have the order of not to be performed, it is necessary not to perform them with one’s will and authority. If those works are not avoided, one must be accountable for them. There are some other works which do not involve our will and authority so, whatever happens is according to our fate. The works having our will and authority for performance will be rewarded and punished accordingly. This study explores the reasons of grief, shock, discernment and difficulties, their reasons and motives in the light of teaching of The Holy Prophet.

Next Generation Sequencing Based Identification and Characterization of Important Genes in Cotton.

Cotton is an economically important crop that generates billions of dollars in annual revenue worldwide. Whitefly (Bemisia tabaci) and whitefly-transmitted cotton leaf curl disease (CLCuD) cause great damage to overall cotton production. Compared to widely cultivated cotton species Gossypium hirsutum, the diploid species G. arboreum is a natural source for resistance against CLCuD. However, the gene expression changes steered by whitefly and CLCuD infestation in G. arboreum and G. hirsutum and the interaction mechanisms of CLCuD to these cotton species remain to be elucidated. In the present study, RNA-Seq based transcriptomics have been subjected to elucidate the differential gene expression in G. arboreum and G. hirsutum under CLCuD infestation. G. arboreum plants were infested with CLCuD by graft and viruliferous whitefly-mediated inoculation. However, for G. hirsutum, CLCuD susceptible and resistant accession Mac7 were infested by whitefly-mediated CLCuD. RNA-Seq was done on plants from each experiment using Illumina HiSeq 2500 and data analysis identified differential gene expression in G. arboreum and G. hirsutum under CLCuD infestation. qRT-PCR, gene ontology enrichment, KEGG pathway analysis and homology searches were used for validation and functional annotation based characterization of identified genes in transcriptomic data in response to CLCuD. Several genes involved in disease resistance and pathogen defense were identified. Furthermore, coexpression network analysis on the RNA-Seq datasets, indicated the hub genes, showing their crucial role in defense or susceptibility of cotton in response to CLCuD. The important gene families identified in cotton under CLCuD stress include phytohormones, transcription factors, kinases, oxidative stress, membrane channel proteins, protein modifications, methylation and metabolism-related genes. Overall, it is concluded that the resulting datasets give inclusive insights into the cotton defense or susceptibility mechanisms under whiteflies and CLCuD infestation. This study reveals several candidate genes involved in a virus-host interaction that might be used for the control of whitefly, other sap-sucking insects and CLCuD in cotton
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