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۱۱۔ پتھریلے شہر

پتھریلے شہر

میں شام کی اداس پگڈنڈی پر دن بھر کے تھکے سورج کی مانند بیٹھا تھا

’’کیوں ‘‘۔۔۔۔۔ ’’کس لیے‘‘

میں اپنے وجود کی نفی کے بارے میں سوچ رہا تھا

منتشر خیالات کا سیلِ رواں رگوں میں محو ِ گردش تھا

ذہن تاریک جھونپڑے کی مانند آزاردہ تھا

شوریدہ سری میری بینائی نگل چکی تھی

میں تصورات کے پتھروںسے ٹھوکریں کھاتا ہوا

اس جھونپڑے میں جا پہنچا جہاں کبھی زندگی ہار دی تھی

اچانک ایک ٹھوکر سے بینائی بحال ہو گئی

English in the Workplace: Business English as a Lingua Franca in Boardwalk Direct Selling Company

With the current international competition among global companies, Business English as a Lingua Franca (BEFL) has become a necessity. As for one, Boardwalk Direct Selling Company recognizes the adoption of the BEFL concept within the organization to equip its workforce with adequate English language skills at par with global standards. This study aims to assess the organization’s current English proficiency and the readiness of its employees to embrace BEFL. This also presents the major English language skills areas that need improvement through training intervention. A stratified sampling method is utilized to extract data via an online survey. Respondents are strategically chosen to represent different strata such as organizational departments or groups, job levels, tenure, and age. A convenient size of 34 respondents participated in this study. Generally, respondents acknowledge the importance of the English language skill set in their job functions and as criteria for their career growth. Half of the sampling population affirms their English language proficiency. However, the study reveals that Boardwalk employees are willing to subject themselves to improving their English skills, most particularly in speaking and writing aspects. Given their willingness, the employees recommend that the company strengthen its BEFL trainings across the organization. Moreover, with the current setup of mostly working from home due to COVID restrictions, majority of the employees prefer online learning.

Molecular and Biochemical Analyses of Cross Adaptation Mechanism S for Salt Tolerance in Rice Oryza Sativa L. Cell Lines

In the present research work cell lines of Oryza sativa cv. Swat-1 were incrementally adapted to osmotic (20 % PEG) and ion toxicity (20 mM LiCl) stresses to study the mechanisms of sequential tolerance and cross adaptation. The adapted cell lines were growing on respective stresses over a period of 55 passages and their tolerance was stable at least up to 6th generation on stress free medium. The physiological characterization of cell lines showed a substantial increase in proline and Ca++ levels and significant reduction in K+ and Mg++ contents on adaptation to PEG stress, while no prominent differences in accumulating organic and inorganic cytosolutes were observed between unadapted and LiCl adapted cell lines. Adaptation to PEG and LiCl was evident by appearance of six different polypeptides, among these four polypeptide bands 15, 17, 26 and 68 kDa were common, while, other two novel proteins 60 kDa, and 32 kDa were unique to PEG and LiCl adapted cell lines, respectively. None of these polypeptide were detectable in unadapted cell lines. At the molecular level the PCR amplification results using seven set of SSR primers for saltol region of chromosome 1 of rice revealed no polymorphism in adapted and unadapted cell lines. When adapted and unadapted cell lines were exposed to 200 mM NaCl with an iso-osmotic effect to 20 % PEG and an osmotic effect 10 times higher than 20 mM LiCl in addition to toxicity effects for two consecutive passages. The tolerance of both adapted lines was similar but significantly greater than tolerance of unadapted cells line. On exposure to subsequent second NaCl stress unadapted cells line failed to survive but the tolerance of adapted cell lines increased, expressing the phenomenon of sequential tolerance. Overall, no qualitative differences were observed among adapted and unadapted cell lines under salt stress. However, the patterns of response of adapted and unadapted cell lines to osmotic and ionic homeostasis to tolerate NaCl were greatly different. PEG adapted cells line appeared to acquire halophytic behavior of a salt accumulator, in contrast LiCl adapted line behaved like a typical salt tolerant glycophyte. Furthermore, the induced tolerance of adapted lines were not restricted to the same type of stress but showed cross tolerance towards other abiotic stresses.
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