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خواجہ عشرت لکھنوی

خواجہ عشرت لکھنوی
افسوس ہے ہماری بزم ادب وشعر طرزقدیم کے اساتذہ شعروسخن سے خالی ہوتی جارہی ہے اورجویہاں سے جاتاہے اپناقائم مقام چھوڑ کرنہیں جاتا۔چند مہینے ہوئے خواجہ عشرت لکھنوی جوبیگماتی اورٹکسالی زبان لکھنے میں اپنی دوایک ہی نظیریں رکھتے تھے، داغ مفارقت دے گئے تھے کہ اب اردو کے ایک مشہور استاد سخن حضرت احسنؔ مارہروی کے انتقال پرمُلال کی خبر آئی ہے۔

 

اسلامی فوجداریت کا ضابطۂ قرائن

Qara’in - usually translated as circumstantial evidence - is a derived form of Arabic word " " قر ن which literally means a fact associated or accompanied with an event or circumstances. But when an event or circumstances discloses such associated or accompanied fact then such a fact becomes circumstantial evidence. Both proto-juristic and modern legal terms held circumstantial evidence for an evidence which is offered to prove certain attendant circumstances from which the existence of the fact at issue may be inferred. In Islamic Law, majority of jurists do not endorse Qara’in as an authoritative evidence, particularly, in offences leading to corporal punishments. On the other side, Ibn Farhun from Malikites and Ibn Qayyem from Hanbalites terms it equal to the direct evidence of Iqrar and Shahadah. It is not very strange that Dr. Anwarullah, a prominent Muslim scholar and Prof. Robert Preach are of the opinion that circumstantial evidence is, after all, more authentic even than the aforesaid two evidences. Herbert Broom- a western legal expert- also says that certain hidden facts can be deducted from the mode of a relevant act or to some extent it is modus operandi which gives birth to a circumstantial evidence. In this shortened article the juristic opinion of some early and contemporary legal experts has been discussed as to judge the legal mode and authenticity of circumstantial evidence.

Evaluation of Synthetic Epsps Gene in Genetically Transformed Plants

Codon optimized synthetic epsps gene was used to develop transgenic tobacco and sugarcane lines using Agrobacterium and biolistic approach, respectively. Putative transgenic tobacco lines analyzed by qRT-PCR showed variable mRNA transcript levels. Southern hybridization performed for determining gene integration patterns showed multiple copy inserts (1-4) of transgene in various tested lines. Immunoblot strips confirmed the translation of cp4-epsps mRNA transcripts in T1 line. Malachite green assay showed variable EPSPS activity levels in various lines in response to glyphosate application. Transgenic tobacco lines exposed to 1% (v/v) glyphosate at 4 to 5 leaf stage showed variable resistance levels ranging from moderate level tolerance to high tolerance. The cp4-epsps mRNA transcript levels varied from genotype to genotype in tested transgenic sugarcane lines regardless of the integration of multiple copy number (3-6) of transgene. Genetic transformation with pyramided constructs results in the integration of multiple genes at a single locus, which is desirable in terms of expression efficiency of transgenes. The cp4-epsps gene was pyramided with insect resistant cry1Ac and cry2Ab genes and cloned in pSb187 vector was used to genetically transform tobacco using Agrobacterium mediated approach. Transgenic lines were confirmed through PCR using gene specific primers of respective genes. The mRNA transcript level of cp4-epsps gene detected through qRT-PCR using GAPDH gene as an internal control was found to be variable among different transgenic lines. Transgenic lines were tested for checking the translational efficiency of all three genes using immunoblot strips. Transgenic tobacco lines found expressing all three proteins were subjected to detached leaf insect bioassays to evaluate the effectiveness of Bt genes in putative transgenic tobacco against insect attack using first instar larvae from Spodoptera littura and Helicoverpa armigera. Transgenic tobacco found to be resistant against these insects showed that cry1Ac and cry2Ab genes were efficient enough in pyramided form. When these transgenic tobacco lines were exposed to glyphosate at 10 to 12 leaf stage, chlorotic symptoms were observed which hinted that herbicide tolerance potential of transgene declined with increasing age of the plants. In order to overcome the spatio temporal gene expression issues, a dual copy epsps expression cassette synthesized under different regulatory elements, cloned in pGreen0029 binary vector was used to generate transgenic tobacco. These tobacco lines exhibited excellent resistance against 19% (v/v) glyphosate at 10-12 leaf stage. This construct has shown tremendous potential regarding transgene expression levels which is required in the field crops. This dual cassette cp4-epsps gene has been recommended for generation of glyphosate resistant cotton and other commercially important cultivars.
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